Fig. 4
The determination of the apparent rate constant (kapp) and the binding constant of TU-1/base pairs. a The plot of the kapp vs. [Base] + [TU-1], in neat PO (14.5 M, 14.5 mmol), [TU-1] = [base] = 0.1, 0.15, and 0.2 M; b The plot of the chemical shifts of two binding systems with the concentration of TU-1 and base, [base] = [TU-1] = 0.01–0.06 M in CDCl3. Keq is the binding constant (at 297 K), and kapp is the apparent rate constant. Errors of kapp and Keq are exactly the errors in the slope of the line, determined from linear regression
