Fig. 3
Comparison of efficiency and product selectivity of HDR vs. base editing following RNP delivery. RNP delivery into HEK293T cells of 200 nM Cas9 or 200 nM BE3 pre-complexed with the S33-targeting sgRNA or an unrelated sgRNA and delivered in a cationic liposome. a Frequency of S33F mutation (blue) and indels (green) from treatment with CORRECT HDR agents (Cas9, sgRNA, and ssDNA donor template) in the ratios shown. b Product selectivity ratio, defined as the ratio of S33F modification to indel modification, resulting from treatment with the CORRECT HDR agents used in a. c Comparison of target C to T conversion efficiency following RNP delivery of BE3 (blue) or CORRECT HDR (purple) and the S33-targeting sgRNA. The control corresponds to cells treated with BE3 protein and unrelated sgRNA. d Product selectivity of base editing and CORRECT HDR. Values and error bars reflect mean ± S.E.M. of three independent biological replicates performed on different days
