Fig. 5
Combination of OxP and LPD-PD-L1 trap gene therapy on orthotopic CT26-FL3 tumor model. a OxP and PD-L1 trap combination treatment scheme. b Tumor growth curves of orthotopic CT26-FL3 tumors in PBS, LPD-pGFP, PD-L1 trap, OxP and OxP+PD-L1 trap gene treated groups (n = 5 mice per group). c TSR% results on day 35. d Mice survival curves. e CD8+ T cells, CD4+ T cells, activated DCs and PD-L1 levels in tumors of mice after various treatments, analyzed by flow cytometry (n = 4). f Masson’s trichrome and immunofluorescence staining of the orthotopic tumors after OxP+PD-L1 trap treatment using DAPI (blue) and anti-CD3 (red). Yellow dotted line indicates the border between intestinal mucosa and the orthotopic tumor. Scale bar represents 50 μm. g Relative mRNA expressions of cytokines in tumors of the OxP+PD-L1 trap treated group compared to PBS group on day 28, detected by quantitative RT-PCR (n = 4). h Th17 cell ratios in the splenocytes of mice after various treatments on day 28 (n = 4). Significant differences were assessed in b using two-way ANOVA with multiple comparisons, in d using log rank test and in c, e, g, and h using t test. Results are presented as mean (SD). ns, not significant. *P < 0.05, **P < 0.01, ***P < 0.001
