Fig. 2 | Nature Communications

Fig. 2

From: A quantitative mass spectrometry-based approach to monitor the dynamics of endogenous chromatin-associated protein complexes

Fig. 2

Application of qPLEX-RIME for the identification of ERα-specific interactors in breast cancer cell lines. a Bar plots illustrating the overlap of the qPLEX-RIME data with known ERα-associated proteins from BioGRID and STRING databases. b Volcano plot summarising the quantitative results of the ERα qPLEX-RIME. ERα and several of its known significantly enriched interactors are labelled. c Sequence coverage of the ERα protein in the qPLEX-RIME analysis. d Boxplots of the number of unique peptides for the overlapping ERα-associated proteins between the non-quantitative RIME and the qPLEX-RIME method. Centre line shows the median, bounds of box correspond to the first and third quartiles and the upper and lower whiskers extend to the largest or the smallest value no further than 1.5 × IQR (inter-quartile range)

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