Fig. 3
From: CRISPR-FRT targets shared sites in a knock-out collection for off-the-shelf genome editing
CRISPR-FRT allows rapid reconstruction and phenotypic characterization of generated mutants. a Mean colistin minimum inhibitory concentrations (MIC) for wild-type MG1655 and otherwise isogenic basR and basS mutants (n = 3; error bars represent the s.d.; two-sample unpaired t-test; ***p < 0.001 vs. wild type). b Mean ciprofloxacin minimum inhibitory concentrations (MIC) for wild-type BW25113 and otherwise isogenic nadC, vacJ and acrR mutants (n ≥ 3; error bars represent standard error of the mean; two-sample unpaired t-test **p < 0.01, ***p < 0.001 vs. wild type). c When treated with amikacin or ciprofloxacin the oppB (A180E) mutant shows a significantly increased surviving persister fraction compared to the wild type (n = 3; error bars represent the s.d.; two-sided t-test; ***p < 0.001). d Mutations in mutL, mutH, uvrD and mfd can change the genomic mutation rate. While all mutants show a higher mutation rate, only the mutL (S101R), mutL (H270R), mutH (W106R) and mfd (V864A) mutants show a significantly higher mutation rate compared to the wild type (n = 24; error bars represent upper and lower limits of the 95% confidence intervals; ShinyFlan R package built-in two-sample comparison [30]; *p < 0.05; ***p < 0.001). e The envZ (L116P) mutant exhibits enhanced growth characteristics in the presence of 5% (v/v) ethanol compared to the wild type (left panel). Both the maximal final density (right top panel) and the growth rate (right bottom panel) significantly improved compared to the wild type (n = 5; error bars represent s.d.; unpaired two-sided t-test; ***p < 0.001). n.s. non-significant
