Fig. 5
From: CRISPR-FRT targets shared sites in a knock-out collection for off-the-shelf genome editing
The distribution of recA alleles encoded by the plasmid pool before CRISPR-FRT is similar to the distribution of recA mutants transferred to the chromosome after CRISPR-FRT. a Column scatter graph showing the distribution of recA mutants encoded by the plasmid pool before CRISPR-FRT and recA mutants on the chromosome after CRISPR-FRT. Mean proportion of mutants shown with heavy bars and standard deviation shown with light bars (n = 31). b Distribution of individual recA mutants before and after CRISPR-FRT. Plotting previously reported12 relative recombination function of recA mutants (circles, second y-axis) suggests deleterious mutants with <1% recombination activity are underrepresented in the original plasmid library pool
