Table 1 CRISPR-FRT efficiency across different genes and methods

From: CRISPR-FRT targets shared sites in a knock-out collection for off-the-shelf genome editing

Gene	Mutation	Rescue DNA	Survivors (CFU’s/ml)	Total screened^a	Passing screens	Sequence confirmed (%)
acrA	D₂₆₈N	PCR product	9	4	2	100
acrR	multiple	PCR product	160	25	13	100
deoC	A₂₂₅V	PCR product	5	4	2	100
malZ	A₁₆₉V	PCR product	39	18	11	100
nadC	Y₆C	PCR product	15	4	3	100
vacJ	L₈F	PCR product	38	4	3	100
ygiD	A₁₂₅T	PCR product	8	4	2	100
ygiV	Q₁₀₄H:L₁₀₅V	PCR product	45	12	10	100
yigI	I₁₀₂T	PCR product	46	9	8	100
basR	G₅₃E	PCR product	31,000	9	8	100
basS	L₁₀P	PCR product	15,000	9	8	100
	C₈₄R	PCR product	2,500	9	8	100
envZ	L₁₁₆P	PCR product	48,000	48	32	100
acrB	S₈₆₃A	PCR product	320	32	19	100
fabF	T₁₃₈A	PCR product	3,000	3	2	100
fabR	V₉₂A	PCR product	13,000	8	8	100
oppB	A₁₈₀E	PCR product	15,000	40	10	100
rbsR	T₁₅A	PCR product	130,000	36	34	100
rob	F₈₂L	PCR product	120	13	6	100
mutL	S₁₀₁R	PCR product	1,160	10	10	100
	H₂₇₀R	PCR product	1,240	10	7	100
mutH	W₁₀₈R	PCR product	200	10	10	100
uvrD	E₆₄₂G	PCR product	140	7	1	100
sspA	H₈₅R	PCR product	5,820	10	7	100
	L₁₆₅P	PCR product	6,620	10	10	100
	T₆₁P	PCR product	9,180	10	4	100
	L₁₅₆P	PCR product	5,780	10	9	100
mfd	V₈₆₄A	PCR product	80	8	2	100
fabA ^b	L₉₀Q	PCR product	37,000	20	5	40
yejM ^b	P₁₂₆S^c	PCR product	3,000	9	8	40
yejM ^b	R₁₆₅C^c	PCR product	37,000	9	9	44
fabF:acrB	T₁₃₈A:S₇₇₅P	PCR product	1,227	36	16	100
fabA:fabR	L₉₀Q:V₉₂A	PCR product	25,200	40	39	100
basR:yejM	G₅₃E:P₁₂₆S^c	PCR product	110,000	18	15	100
basS:yejM	C₈₄R:R₁₆₅C^c	PCR product	40,000	18	12	100
yehL	S₉₀P	plasmid	2,000	12	12	100
recA	multiple	plasmid	350,000	100	77	100
traT	FLAG tag	PCR product	240	24	9	100

^aClones assayed for Kan^S (noSCAR system only) and yielding correct PCR fragment size
^bEssential gene; neighboring, non-essential gene targeted
^cD₃₄₇G:G₃₄₈S yejM mutations present in founder strain were also recovered

Back to article page

Table 1 CRISPR-FRT efficiency across different genes and methods

Search

Quick links