Fig. 1
From: Helicase promotes replication re-initiation from an RNA transcript
Non-replicating DNAP prevents helicase slippage and increases unwinding rates. a Cartoon illustrating T7 helicase unwinding and slippage behavior. The helicase unwinds, loses grip, slips, re-grips, and resumes unwinding. Arrows indicate the directions of helicase movements. b, c Representative traces showing the number of unwound base pairs by T7 helicase vs. time in the absence (28 traces in total) or presence (25 traces in total) of non-replicating DNAP, respectively. Experiments were conducted with 2 mM ATP under 8 pN force. For clarity, traces have been arbitrarily shifted along both axes. d Measured processivity of T7 helicase (mean distance between slippage events) in the absence and presence of gp5 and/or trx with 2 mM ATP under 8 pN external force. e T7 helicase unwinding rates between slips as a function of force in the absence or presence of the DNAP with 2 mM ATP. Unwinding rates were determined between slippage events (if any). Error bars represent standard deviations
