Fig. 5
H3v-47 localizes to interfaces between virus and cell surface or between viral particles. a TEM images of the surface of MDCK cells inoculated with A/Minnesota/11/2010 H3N2v virus and infected cells were incubated with mAb H3v-47, zanamivir, mAb CR8020, or plain Opti-MEM at 3 h postinoculation and fixed for imaging at 14 h. For each image, the virus particles in the red squares are shown at higher magnification, below. Representative images of two independent experiments are shown. The white scale in each image represents 500 nm. b TEM images are shown of the surface of MDCK cells that were inoculated with virus and subsequently incubated with H3v-47 mAb similar to a, with addition of anti-human IgG conjugated to 10 nm gold particles at 13 h post-inoculation. The black opaque dots indicated by the red triangles represent the gold particles. The white scale in each image represents 100 nm. The experiment was conducted twice independently
