Fig. 8

Dendrite defects in hippocampal neurons of Nek7^gt/gt mice. a GFP-expressing 14 DIV hippocampal wild-type and Nek7^gt/gt neurons. Scale bar, 50 μm. b Total dendrite length in neurons as in (a); n = 8 wild-type and 7 Nek7^gt/gt embryos. Total number of neurons: 139 (wild type) and 84 (Nek7^gt/gt). ***P < 0.001, by two-tailed t-test. c Mean number of intersections by Sholl analysis of neurons as in (a); n = 126 wild-type and 71 Nek7^gt/gt neurons. **P < 0.01, ****P < 0.0001 by two-tailed t-test. d Spines in primary dendrites of neurons as in (a). Scale bar, 1 μm. e Density of spines as in (d); n = 15 wild-type and 10 Nek7^gt/gt neurons, 3 embryos per genotype, 552 and 260 spines, respectively. *P < 0.05, by two-tailed t-test. f Head diameter of spines as in (d). Statistics as in (d). g Kymographs of EB3 comets in dendrites of wild-type or Nek7^gt/gt 9 DIV neurons. White arrowheads indicate retrograde growth. h Comet density in dendrites of wild-type or Nek7^gt/gt neurons. i Percentage of retrograde comets in the segments analyzed in (h). h, i n = 3 wild-type and 3 Nek7^gt/gt embryos, 26 and 25 dendrites, respectively. n.s. non-significant, *P < 0.05 by two-tailed t-test. j Maximum intensity projections of diolistically labeled dendritic trees of CA1 wild-type or Nek7^gt/gt neurons in 4-month-old mice and representative tracings. Scale bar, 25 μm. k Total length of dendrites shown in (j); n = 3 wild-type and 3 Nek7^gt/gt mice (2 males and 1 female each), 54 neurons per genotype. *P < 0.05 by two-tailed t-test. l Mean intersection number in dendrite trees shown in (j). Number of animals as in (j), 41 and 44 neurons per genotype, respectively. *P < 0.05 by two-tailed t-test. m Dendritic spines in primary dendrites of neurons as in (j). Scale bar, 1 μm. n Head diameter of spines as in (m). o Density of spines as in (m); n = 12 wild-type and 11 Nek7^gt/gt neurons, 3 embryos per genotype, 1263 and 823 spines, respectively. *P < 0.05, by two-tailed t-test. Error bars: s.e.m. Columns in all graphs show means and dot overlays individual data points