Fig. 2

cMyc, but not HIF1α, is required for NK cell effector functions. a–d cMyc^−/− (cMyc^flox/flox × Tamox-Cre) or WT (cMyc^WT/WT × Tamox-Cre) NK cells were left unstimulated (US) or were stimulated for 18 h with IL-2/IL-12 before flow cytometry analysis for % IFNγ-positive NK cells and mean fluorescence intensity (MFI) of IFNγ expression in IFNγ⁺ NK cells, and granzyme B expression. e–h Hif1α^−/− (Hif1α^flox/flox × Vav-Cre) or WT (Hif1α^flox/flox) NK cells were left unstimulated or were stimulated for 18 h with IL-2/IL-12 before flow cytometry analysis for percent IFNγ-positive NK cells and granzyme B expression. Data are mean ± s.e.m. or representative of five independent experiments. Statistical analysis was performed using Student's t-test (b, f) or a one-sample t-test vs. a theoretical value of 1 (b–h); *p < 0.05, **p < 0.01, ns non-significant