Fig. 4
From: Global pairwise RNA interaction landscapes reveal core features of protein recognition
Mutational trajectories between two natural binding elements. a Predicted outcomes from mutations that bridge the Box B left and Box B right motifs. A Hamiltonian score estimates the predicted outcome from each mutation and each possible change at six positions is illustrated on a disc with five concentric circles radiating from Box B right. Light colors suggest favorable mutations while dark colors represent disruptive mutations that might block the evolutionary pathway according to the color scale bar. Two extreme pathways are labeled by dashed arrows (black for most favorable mutational pathway, white for least favorable mutational pathway). b Evaluation of mutational pathways in cells using the yeast-three hybrid assay (see Supplementary Data 1). Mutational pathways are represented with secondary RNA structures (upper for most favorable pathway, lower for least favorable pathway). Bars depict experimental outcomes. c Rotation of a provides a three-dimensional representation potential mutational trajectories. Valleys identify less favorable pathway while peaks suggest favorable ones (error bars represent 1 standard deviation, three or more biological replicates corresponding to separate cultures were conducted for each measurement)
