Fig. 1
From: Urinary cell-free DNA is a versatile analyte for monitoring infections of the urinary tract
Shotgun sequencing assay and biophysical properties of urinary cfDNA. a Schematic representation of the ssDNA library preparation protocol used for shotgun sequencing of urinary cfDNA20. Key steps include: (i) cfDNA isolation, (ii) DNA denaturation, (iii) ssDNA adapter ligation, (iv) extension and double-stranded DNA adapter ligation, and (v) PCR. b Overview of post-transplant sample collection dates (color indicates pathology, bars connect samples from same subjects). c, d Fragment length density plot measured by paired-end sequencing for different cfDNA types: c chromosomal and BKV cfDNA from representative samples, and d E. coli, parvovirus B19, and mitochondrial cfDNA from representative samples. Fourier analysis reveals a 10.4 bp periodicity in the fragment length profiles of chromosomal and BKV cfDNA but not for E. coli, parvovirus B19, or mitochondrial cfDNA (insets). See Supplementary Data 1
