Fig. 2
From: Biosynthesis of thiocarboxylic acid-containing natural products
Proposed biosynthetic pathway and in vitro characterization of thiocarboxylic acid biosynthesis. a 5-SH is the enzymatic product of the transformation of 5 by the thioacid cassette, PtmA3 and PtmU4, capturing a sulfur atom from the sulfur-carrier protein machinery. The C-terminal GG motif and capped residues (MV in PtmS2) are highlighted in red and blue, respectively. SCP sulfur-carrier protein (circled), ASA aspartate semialdehyde, DHAP dihydroxyacetone phosphate, AHBA (6) 3-amino-4-hydroxybenzoic acid, ADHBCoA (5-CoA) S-(3-amino-2,4-dihydroxybenzoate) coenzyme A. b ThioPTM (1) and thioPTN (2) result from the coupling of 5-SH with platensicyl- and platencinyl-CoA, respectively. c UV at 260 nm from HPLC analysis of in vitro PtmA3 reactions with 5 and 6. std standard. d UV at 260 nm from HPLC analysis of in vitro PtmU4 reactions with 5-CoA using the native sulfur donor. Sodium thiosulfate (Na2S2O3) was used as the inorganic sulfur source
