Table 1 Summary of the steady-state kinetics of PtmA3

From: Biosynthesis of thiocarboxylic acid-containing natural products

Substrate

Km (μM)

Ki (μM)

Kcat (min−1)

rel Kcat Km−1

5

22.9 ± 3.0

184 ± 24

14.1 ± 0.9

1

6

470 ± 60

638 ± 89

23.8 ± 2.2

0.082

7

(1.24 ± 0.11)×103

n.a.

6.89 ± 0.3

0.009

8

70.9 ± 5.0

n.a.

1.03 ± 0.02

0.024

9

290 ± 21

n.a.

1.39 ± 0.03

0.008

10

322 ± 15

n.a.

15.8 ± 0.3

0.079

  1. All experiments were performed in triplicate and the data are listed with standard deviations The relative rates are compared to the native substrate 5. n.a. not applicable
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