Fig. 2
From: An anionic phthalocyanine decreases NRAS expression by breaking down its RNA G-quadruplex
ZnAPC induces ROS production in vitro and in cells. a Fluorescence spectra of 10 μM HPF as an ROS indicator in the presence of 2 μM ZnAPC, FeAPC, NiAPC or CuAPC at 25 °C. Excitation wavelength was 490 nm. The spectra at irradiation time points 0 and 120 min are highlighted in black and red, respectively. b Plots of the change in fluorescence intensity of HPF at 515 nm during the photo-irradiation. c Evaluation of ROS levels by means of HPF in MCF-7 cells treated with ZnAPC or FeAPC after photo-irradiation for 1 h. DIC images of cells and fluorescent images of HPF are presented. Scale bar, 20 µm. d The mean fluorescence intensity of HPF was quantified. Relative fluorescence intensities are shown, and each bar represents mean ± SD for 10 images. e Cells pre-treated with ZnAPC or FeAPC for 1 h were incubated for 24 h after photo-irradiation for 2 h. The number of viable cells was determined by Trypan Blue exclusion–based cell staining. The number of treated cells was normalised to that of untreated cells. Each bar represents mean ± SD; n = 3. All intracellular experiments were conducted with 10 μM HPF, 10 μM ZnAPC or 10 μM FeAPC
