Fig. 6

Energy transfer from ZnAPC to NRAS RNA is greater than that to NRAS DNA. a Fluorescence spectra of 2 μM ZnAPC under the following experimental conditions: in the absence of NRAS RNA in the buffer (right blue) and in the buffer containing 30 wt% DMF (dark blue), in the presence of NRAS RNA in the buffer (red) and in a buffer containing 30 wt% DMF (brown), in the presence of NRAS DNA in the buffer (light green), and in the buffer containing 30 wt% DMF (dark green). b Fluorescence decay of ZnAPC under the experimental conditions listed in a. Fluorescence decay in the buffer is not shown because of the insufficient fluorescence intensity. All the experiments were conducted with 2 µM ZnAPC, 10 µM NRAS RNA, and 10 µM NRAS DNA. c Fluorescence lifetime, τ, was evaluated with a theoretical equation of single or double exponential decay for the fluorescence decay obtained in b