Fig. 2

Pregnant macaques inoculated IV and IA develop prolonged ZIKV viremias and ZIKV RNA in amniotic fluid but show no fetal head growth restriction. a Viremia and ZIKV RNA levels in plasma and amniotic fluid of ZIKV-infected pregnant rhesus macaques: Each line shows ZIKV RNA (solid lines) or infectious virus (dotted lines) kinetics for a single animal, detected in mean log₁₀ RNA copies assayed in triplicate with error bars showing standard deviations or mean log₁₀ Vero cell plaque forming units (PFU) per ml assayed in duplicate. The horizontal solid line shows the ZIKV RNA limit of detection (LOD) of 2.0 log₁₀ RNA copies and the horizontal dotted line shows the plaque assay LOD of 1.6 or 1.9 log₁₀ PFU per ml. For the GD64 mother, due to recurring placental bleeding, amniotic fluid was no longer collected after 56 dpi (GD 120). b–d Most ZIKV-infected fetuses show normal growth as measured by head size determined by b the biparietal diameter at weekly ultrasound compared to the CNPRC colony mean (black line) ±2 s.d. (upper and lower gray lines), which was based on 10–100 fetuses reported earlier⁶⁰, c computed tomography (CT) scans at GD 141–148 and d relative head size calculated as the biparietal diameter divided by the femur length