Fig. 3

ZIKV infection associates with early death of GD41 fetus. a Schematic representation of infection outcome that resulted in fetal death 7 dpi. b ZIKV RNA levels in maternal fluids sampled by venipuncture, cystocentesis, saliva eluted from cotton swabs placed in the cheeks of macaques, or cervical spinal tap, reported in mean log₁₀ RNA copies/ml and assayed in triplicate with error bars showing standard deviations. The dotted line shows the LOD, 2.3 log₁₀ RNA copies/ml. c ZIKV RNA and infectious virus in placental and fetal tissues 7 dpi (except where noted for amniotic fluid), detected in mean log₁₀ RNA copies assayed in triplicate or log₁₀ Vero cell plaque forming units (PFU) per ml or gram tissue. The dotted lines show the ZIKV RNA LOD of 1.3 log₁₀ RNA copies or the plaque assay LOD of 1.6 log₁₀ PFU per ml or gram tissue. Amniotic fluid from 2 dpi was not tested (nt) by plaque assay. Error bars on RNA measures show standard deviations for three replicates. d Hematoxylin and eosin and in situ hybridization images of selected fetal tissues showing ZIKV RNA labeled brown and highlighted with arrows. Scale bars are as follows: heart, 54 μm, cornea, 150 μm, placenta villi, 130 μm, placental disc, 200 μm