Fig. 3
From: Anti-phage islands force their target phage to directly mediate island excision and spread
ICP1-encoded PexA is necessary and sufficient for PLE cirularization. a miniPLE circularizes in the absence of ICP1 infection upon ectopic expression of PexA. b miniPLE does not circularize during ICP1 infection when pexA is knocked out. c Western blot for PexA during ICP1 infection. PLE− V. cholerae cultures were probed for PexA at the listed times after infection with ICP1 or ICP1 ΔpexA. To determine if PexA is packaged in the phage particle, 5 times the PFU of phage as was used for infection was probed for the presence of PexA. Purified PexA (20 ng) was used as a positive control. Uncropped gels are presented in Supplementary Fig. 5
