Fig. 5
From: Anti-phage islands force their target phage to directly mediate island excision and spread
PexA directs historic PLEs to circularize and is dispensable for PLE 1 anti-phage activity. a Circularization PCR of all PLEs probed during ectopic expression of PexA. b Fold change in PLE 1 copy number as measured by qPCR. Left, PLE copy number was compared before and 20 min after ICP12004 or ICP12004 ΔpexA infection. Right, PLE copy number was examined before and 15 min following ectopic expression of an empty vector (EV) or pexA. Bars represent the mean and standard deviation of three independent replicates tested (ns, not significant by T Test). c Tenfold dilutions of ICP1 spotted on V. cholerae PLE− or PLE 1 lawns showing the ability of different phage strains to form plaques (dark spots, zones of killing). ICP1 and ICP1 ΔpexA are unable to form plaques on PLE 1, while the CRISPR proficient phage is able to overcome PLE 1 and form plaques18. Uncropped gels are presented in Supplementary Fig. 5
