Fig. 5
From: Cell entry of a host-targeting protein of oomycetes requires gp96
Molecular tweezers inhibit the translocation of SpHtp3. a Superimposition of the 10 lowest-energy NMR-based structures of the C-terminal peptide of SpHtp3 with the central helix (P204-K211) highlighted. b Electrostatic surface presentation of the C-terminal peptide of SpHtp3. Positive, neutral and negative charges are displayed in blue, grey and red, respectively. c Superimposition of the NMR-based structures of the C-terminal peptide wt (blue) and the double mutant (K208A/R210A, red) of SpHtp3 with the central helix (P204-K211). d 1H-1D NMR titration experiments of the SpHtp3 peptide with a stepwise increasing amount of tweezers as indicated. Decreasing signal intensities indicate an interaction of both. e Effect of molecular tweezers on the translocation of SpHtp3-mRFP into RTG-2 cells. With increasing tweezers’ concentrations, the uptake and cell surface binding of SpHtp3 are interrupted. Nuclei are indicated by dashed lines. Error bars denote s.e.m. (cells: 50). ***p < 0.001 (one way ANOVA). Scale bar: 20 µm (n = 3)
