Fig. 1

Uhrf1 deficiency disrupts bivalent histone modification. a Distribution of Uhrf1-enriched regions in gene body and intergenic regions. b Comparative analysis of up-regulated (left) and down-regulated genes (right) between Uhrf1 and Dnmt1 KO. Representative genes and over represented GO terms for unique and common genes are also shown. Dashed line represents 0.05 FDR cut-off. c Comparative analysis of H3K4me3, H3K27me3, and H3K9me3 change between Uhrf1-enriched (e) and Uhrf1-depleted (d) regions. Y-axis represents the difference of log₂(ChIP/input) between WT and Uhrf1 KO. d Changes of promoter classes between WT and Uhrf1 KO ESCs. e Histone modification landscape of representative genes related to pluripotency, mesoderm/mesenchyme/muscle, and neuroectoderm development. f GSEA for stem cell functions. Statistical enrichment (−log₁₀(FDR)) in Uhrf1 KO and WT was shown by blue and red color, respectively