Fig. 6

Intra-nodule and regional heterogeneity in doxorubicin chromatin binding. CEM FLIM data from murine cancer model based on IGROV-1 cells with mice treated with 5 mg kg⁻¹ doxorubicin doses by IP injection. a, b, c show intra-nodule variation in normalized histograms of H1-GFP FRETing population fractions 3 h after IP delivery of drug, together with histograms of estimated intracellular doxorubicin concentration, d, e, f for three fields-of-view of a tumor nodule (T1) on the peritoneal wall, a tumor nodule (T2) on the spleen, and a second tumor nodule (T3) on the spleen in the same mouse, respectively. g, h, i show the corresponding maps of the H1-EGFP FRETing population fraction (where color scale from blue to red spans 0–1). j shows normalized histograms for H1-GFP FRETing population fractions and k shows estimated intracellular doxorubicin concentration for 6 fields-of-view from a single nodule in a control mouse and l shows the corresponding map of the H1-EGFP FRETing population fractions. m shows normalized histograms for H1-GFP FRETing population fractions and n shows estimated intracellular doxorubicin concentration for 6 fields-of-view from a single nodule in a mouse, 3 h after intra-peritoneal delivery of 5 mg kg⁻¹ doxorubicin and o shows the corresponding map of the H1-EGFP FRETing population fraction. For all images the scale bar is 120 μm