Fig. 3
From: Out-of-equilibrium microcompartments for the bottom-up integration of metabolic functions
Coupling metabolic and cofactor regeneration modules. a Reaction network. The G6PDH activity is coupled to the IMVs activity for NADH constant recycling. b G6P dependency. NADH concentration versus time (t) of solutions containing NADH (500 μM), IMVs (1.3 × 109 vesicles per mL), G6PDH (0.1 U mL−1) and increasing concentrations of G6P (0, 0.1, 0.2, 0.4, 0.6, 0.8, and 1 mM). Control solution without IMVs in black. The graph below shows the dependency of the lifetime of the reaction (τ1) toward G6P concentration. c IMVs dependency. NADH concentration versus time (t) of solutions containing NADH (500 μM), G6P (0.8 mM), G6PDH (0.5 U mL−1) and increasing concentrations of IMVs (0.9 × 107, 1.8 × 108, 3.6 × 108, 7.2 × 108, 14.5 × 108, 2.9 × 109, and 5.8 × 109 vesicles per mL). The graph below shows the dependency of the lifetime of the reaction (τ2) toward IMVs concentration. d Enzyme dependency. NADH concentration versus time (t) of solutions containing NADH (500 μM), IMVs (5.4 × 108 vesicles per mL), G6P (0.8 mM) and increasing concentrations of G6PDH (0, 0.0008, 0.004, 0.006, 0.0086, 0.013, 0.02, and 0.5 U mL−1). Control solution without IMVs in black. Reactions are performed in NaOH-Tricine buffer (100 mM, pH 8.0) with MgCl2 5 mM. Error bars are defined as s.d. (N = 3)
