Fig. 6

Loss of miR-143 and miR-145 results in slight reduction in long-term survival. a Kaplan–Meier survival curve of wild-type (WT, n = 29), miR-143/145^−/− (n = 20), miR-146a^−/− (n = 38), and miR-143/145/146a^−/− (n = 34) mice. Corresponding P values are indicated in the table. b, c Micrographs of H&E-stained marrow (b) and spleen (c) from WT, miR-146a^−/−, miR-143/145^−/−, and miR-143/145/146a^−/− mice (scale bar: 50 μm). Hypolobated megakaryocytes (arrow) were distributed through the marrow of miR-143/145^−/− mice, while miR-146a^−/− and miR-143/145/146a^−/− marrows revealed extensive fibrosis. Extramedullary hematopoiesis was evident in miR-146a^−/− and miR-143/145/146a^−/− spleens (arrows indicate megakaryocytes), while miR-143/145^−/− mice showed normal splenic architecture