Fig. 7

Long-term miR-143-145 deficiency results in myeloid expansion. a White blood cell (WBC), hemoglobin (HGB), and platelet (PLT) counts in peripheral blood of 80-week-old wild-type (WT; n = 28) and miR-143/145^−/− mice (n = 23). b Micrographs of spleen sections from WT and miR-143/145^−/− mice immunostained for Mac1 (CD11b) (scale bar: 50 μm). Insets show higher magnification of selected area (scale bar: 100 μm). c Microscopic images of liver sections from WT and miR-143/145^−/− mice immunostained for CD11b (scale bar: 50 μm). Insets show higher magnification of selected area (scale bar: 100 μm). d–g Linear regression and Pearson's correlation analysis was performed on data from aged miR-143/145^−/− mice (82–101 weeks old) to compare the relationship between spleen/liver weights and WBC count (d, e, n = 17), spleen and liver weight (f, n = 17), and WBC count and the number of progenitors (Lin⁻Sca1⁻c-Kit⁺) (g, n = 8)