Fig. 4
On-time removal of the LE is defective in LIF-replaced glandless PgrCre/+Foxa2f/f mice. Control, gland-containing LtfiCre/+Foxa2f/f and glandless PgrCre/+Foxa2f/f mice received i.p. injections of recombinant mouse LIF on GD 4 and were evaluated at 2000 hours on GD 5. a Section of implantation sites on GD 5 were stained with hematoxylin and eosin. Representative images are shown for two independent mice representing observed variation. Columns 1 and 3—Scale bar: 250 μm; Columns 2 and 4—Scale bar, 50 μm. b Immunofluorescence analysis of CDH1 in implantation sites. Areas of breached epithelium are indicted with white arrowheads. Row 1—Scale bar: 100 μm; Row 2—Scale bar: 50 μm; Row 3—Scale bar: 10 μm. c Immunofluorescence analysis of CLDN1 in implantation sites. Areas of breached epithelium are indicted with white arrowheads. Scale bar: 50 μm. AM: antimesometrial, M: mesometrial, Em: embryo, LE: luminal epithelium. All images are representative of three independent experiments
