Fig. 5
From: ATR is a multifunctional regulator of male mouse meiosis
ATR regulates DSB marker counts during zygonema and pachynema. a, b RPA (magenta) and SYCP3 (green) immunostaining in Atrfl/+ and Atrfl/– males. c RPA, RAD51 and DMC1 counts at mid zygonema in Atrfl/+ males (n = 2 males; 16 cells for RPA, 15 cells for RAD51, 15 cells for DMC1) and Atrfl/– males (n = 2 males; 16 cells for RPA, 15 cells for RAD51, 15 cells for DMC1). d, e Examples of early pachytene cells from Atrfl/+ and Atrfl/– males with normal autosomal synapsis. Two representative autosomes (aut 1 and 2) are boxed in upper panels and magnified in lower panels. f autosomal RPA, RAD51 and DMC1 counts at early pachynema in Atrfl/+ males (n = 2 males; 49 cells for RPA, 19 cells for RAD51, 33 cells for DMC1) and Atrfl/– males (n = 2 males; 43 cells for RPA, 19 cells for RAD51, 35 cells for DMC1). g, h Early pachytene Atrfl/+ and Atrfl/– cells with normal autosomal synapsis and the X chromosome (boxed in upper panels) identified by Slx DNA FISH (dashed circles in lower panels). i Early pachytene Atrfl/– cell with autosomal asynapsis. j X-chromosome RPA, RAD51 and DMC1 counts at early pachynema in Atrfl/+ males (n = 2 males; 64 cells for RPA, 49 cells for RAD51, 59 cells for DMC1) and Atrfl/– males (n = 2 males; 62 cells for RPA in autosomal synapsis category, 35 cells for RPA in autosomal asynapsis category, 36 cells for RAD51, 53 cells for DMC1). Mean and p values (Mann–Whitney test) indicated. Scale bars 10 µm
