Fig. 2

Pharmacological inhibition of Ezh2 methyltransferase inhibits metastasis in vivo. a Schematic of the preclinical model. b Representative images of hematoxylin and eosin stained paraffin-embedded lungs from mice treated with vehicle control (Captisol) or GSK-126 (150 mg/kg) via intraperitoneal injection, three times a week. Mice were killed after 6 weeks on water supplemented with doxycycline. Enumeration of metastatic lung lesions in drug treated cohorts as outlined in the schematic, or in Ezh2+/+ or Ezh2−/− Tet-ON PyVmT mice induced with doxycycline for 6 weeks. Scale bar is 5 mm. *p < 0.05, two tailed t-test. c Schematic of orthotopically injected PyVmT cells in an immune-competent FVB strain host. GSK-126 or Captisol vehicle control treatment commenced when tumours reached 5×5 mm. d Enumeration of lung lesions from mice treated with GSK-126 or vehicle for 24 days. *p < 0.05 **p < 0.01, two tailed t-test. Met. penetrance, penetrance of metastatic lung lesions