Table 1 Isothermal titration calorimetry of protein–RNA interactions
Binding partners | N (protein:RNA) | KD (10−6 M) | ∆H (104 cal/mol) | ∆S (cal/mol/deg) |
|---|---|---|---|---|
RRM1 + 7-mer (AGUAGAU) | 1.01 | 20.4 ± 1.06 | −1.99 ± 0.07 | −45.2 |
RRM2 + 7-mer (AGUAGAU) | 1.09 | 6.8 ± 0.14 | −1.64 ± 0.006 | −31.6 |
UP1 + 7-mer (AGUAGAU) | 0.75 | 3.4 ± 0.12 | −3.53 ± 0.04 | −93.4 |
UP1 + 17-mer(A35C) | 1.07 | 3.1 ± 0.19 | −1.98 ± 0.03 | −41.2 |
UP1 + pri-mir-18a | 1.35 | 0.1477 ± 0.0089 | −3.52 ± 0.02 | −86.8 |
UP1 + 12-mer (AGUAGAUUAGCA) | 1.01 | 0.0155 ± 0.0034 | −3.81 ± 0.02 | −92.0 |
UP1 + 12-mer-mut1 (AGUUUAUUAGCA) | 1.06 | 0.1541 ± 0.011 | −2.97 ± 0.02 | −68.6 |
UP1 + 12-mer-mut2 (AGUAGAUUUUCA) | 1.08 | 0.330 ± 0.0265 | −2.45 ± 0.02 | −52.5 |
UP1 + 12-mer-mut3 (UUUAGAUUAGCA) | 0.98 | 0.00833 ± 0.00135 | −4.10 ± 0.02 | −101 |
UP1 + 10-mer (UAGAUUAGCA) | 1.07 | 0.01912 ± 0.00673 | −3.94 ± 0.04 | −96.8 |
UP1(R75E/R88A) + 12-mer (AGUAGAUUAGCA) | 1.08 | 0.0190 ± 0.00205 | −3.84 ± 0.02 | −93.5 |
UP1(R75E/R88E) + 12-mer (AGUAGAUUAGCA) | 1.1 | 0.0401 ± 0.0044 | −3.68 ± 0.02 | −89.5 |
