Fig. 2
From: Crystal structure of the catalytic domain of HIV-1 restriction factor APOBEC3G in complex with ssDNA
Antiviral restriction activity of FLAG-NTD-CTD2. a Representative western blot showing 293T cells co-transfected with increasing amounts of wild-type FLAG-A3G or FLAG- NTD-CTD2 (21, 42, 84, 170 ng; black triangles), HDV-EGFP, and VSV-G in the presence or absence of Vif-HA. Percent A3G expression is shown for each respective lane. Single-cycle infectivity of Vif+ (b) and Vif− (c) HDV-EGFP virus prepared in the presence of increasing amounts of FLAG-A3G (black bars) or FLAG-NTD-CTD2 (light gray bars) assayed in TZM-bl target cells. Data reflects the average relative light units (RLU) normalized to the no A3G control. Error bars represent the standard deviation for three independent experiments
