Fig. 1
From: Mouse MRI shows brain areas relatively larger in males emerge before those larger in females
Scanning apparatus, and scanned mice (S) vs. non-scanned littermates (L) data. a Custom 3D printed holders for neonatal mice. b Up to seven mice at a time were scanned using a saddle coil array. c Body weight was not significantly different between scanned and unscanned mice (\(\chi _2^2 = 0.13\), P = 0.94). There was also no interaction effect of scanning and sex (\(\chi _1^2 = 0.097\), P = 0.75). Trendlines and bars for standard error were calculated using centered linear mixed-effect models. d Repeated scanning did not have a significant effect on puberty onset (\(F_{3,64} = 24.67\), t64 = 1.56, P = 0.12) but did have a significant effect on weight at puberty (\(F_{3,64} = 10.67\),\(t_{64} = - 2.43\), P = 0.02). However, neither measure had a sex-scanning interaction (\(t_{64} = - 0.045\), P = 0.96 and \(t_{64} = 0.451\), P = 0.65). e Scanning did not have a significant effect on the levels of sex hormones estradiol (\(F_{3,48} = 1.11\),t48 = 0.16, P = 0.87), testosterone (\(F_{3,48} = 4.17\), t48 = 0.32, P = 0.15), LH (\(F_{3,48} = 0.68\), t48 = 1.03, P = 0.31), FSH (\(F_{3,48} = 14.19\),t48 = 0.28, P = 0.78). f Organ weight of testes (\(F_{1,18} = 1.05\), \(t_{18} = - 1.0\), P = 0.32), ovaries (\(F_{1,28} = 0.03\), t28 = 0.17, P = 0.87), and uteri (\(F_{1,28} = 0.17\), \(t_{28} = - 0.41\), P = 0.69) were not affected by repeated scanning. Means and bars for standard error for d, e, and f were estimated using linear models
