Fig. 3
From: Embryos and embryonic stem cells from the white rhinoceros
Isolation of inner cell mass and derivation/characterization of embryonic stem cell lines. a Isolated ICM and trophoblast from rhinoceros embryo after mechanical dissection with insulin needles (40×); b ICM outgrowth 48 h after plating on feeder cells (20×). c Representative image of ES colonies 24 h after enzymatic passaging; d ES colonies ready for the next passaging. Characterization of undifferentiated ES colonies: e, i, o OCT4 staining; f, l, p NANOG staining; g, m, q SOX2 staining; h, n, r SSEA3 staining. Characterization of differentiated derivatives: s p75 staining of neural crest precursors; t, u Tuj1 and TH staining of neuronal cells; v, z, x GATA4, GATA6, SOX17 staining of endodermal cells; y, w MYOSIN, NKH2.5, TNNT2 staining of myocardial cells. Bar 100 µm; j RT-PCR for SOX2, OCT4, and NANOG in fibroblasts and rESC (n = 1 sample for each cell line)
