Fig. 3
From: Evolutionary trade-offs associated with loss of PmrB function in host-adapted Pseudomonas aeruginosa
ΔpmrB downregulates CFTR expression on airway epithelial cells. a CFTR expression on uninfected (red), LESB65 infected (black) and ΔpmrB infected (blue) A549 airway epithelial cells as measured by flow cytometry and compared to isotype control staining (grey). CFTR alexa fluor 488 median fluorescent intensity (MFI) on stained (b) A549 and (c) Detroit cells. Data in (b) are a composite of two independent experiments with two-three biological replicates per condition in each experiment. Data in (c) are from a single experiment containing 4 biological replicates. Asterisks (*) represent significant differences in one-way ANOVA with Dunnett’s multiple comparison test. *p < 0.05, ***p < 0.001, ****p < 0.0001
