Fig. 4
From: Evolutionary trade-offs associated with loss of PmrB function in host-adapted Pseudomonas aeruginosa
Cif and Ivy contribute to establishment of lung infection. Mice were infected by intranasal instillation of 1 × 106 colony forming units (CFU) P. aeruginosa PAO1, or PAO1 with transposon insertions in cif or ivy. Mice were culled immediately after infection (n = 4 per group) or at 1, 3 and 7 days post-infection (n = 6 per group) and CFU in (a) lung and (b) nasopharynx determined by plating of tissue homogenates onto selective agar. Data are from a single experiment. *p < 0.05, **p < 0.01, ***p < 0.001 in two-way ANOVA with Dunnett’s multiple comparison test vs. PAO1
