Fig. 6
MGLL-CB2 axis regulates tumor progression in mice. a Relative mRNA levels of TNFα, IL-6 and IFNγ in the CD8+ T cells in the MC-38 tumors inoculated subcutaneously in WT, TgMGLL, TgCB2, and TgMGLL+CB2 mice. The data represent the means ± s.e.ms. (n = 5, ***P < 0.005, Student’s t-test; ns not significant). b Six-week-old WT, TgMGLL, TgCB2, and TgMGLL+CB2 mice were subcutaneously inoculated with MC-38 cells, and the tumor volume was measured dynamically. This experiment was repeated three times. The data represent the means ± s.e.ms. (n = 10, **P < 0.01, ***P < 0.005, Student’s t-test). c The survival time of the MC-38 tumor-bearing mice. The mice were described in b. The data represent the means ± s.e.ms. (n = 10, **P < 0.01, ***P < 0.005, Gehan-Breslow-Wilcoxon test). d The incidence of tumor metastasis in MC-38 tumor model. Six-week-old WT, TgMGLL, TgCB2, and TgMGLL+CB2 mice were intravenously injected with MC-38 cells via the tail vein, and the metastases in the lungs and livers were checked 3 weeks later. This experiment was repeated three times. The data represent the means ± s.e.ms. (n = 6~10, *P < 0.05, Student’s t-test). e H&E staining of the lungs from the MC-38 tumor-bearing WT, TgMGLL, TgCB2, and TgMGLL+CB2 mice as described in d.The blue dotted lines indicate the tumor lesions. Scale bars, 200 μm. f Relative tumor lesions were calculated. The data represent the means ± s.e.ms. (n = 9, ***P < 0.005, Student’s t-test). g Volume of tumors from WT mice implanted with MC-38 cells admixed 2:1 with macrophages isolated from corresponding tumors grown in WT, TgMGLL, TgCB2 or TgMGLL+CB2 mice. The data represent the means ± s.e.ms (n = 5, ***P < 0.005, Student’s t-test; ns not significant)
