Fig. 1

RNA destabilization in ALS fibroblasts. a Schematic of Bru-seq (top) and BruChase-seq (bottom). BrU Bromouridine, Anti-BrU antibodies that recognize BrU. Example traces of RNA transcripts destabilized (b) or stabilized (c) in C9ALS fibroblasts. Blocks and lines denote gene and transcript structure, respectively. + strand genes are in green, – strand genes are in red. The traces represent RNA abundance at 0.5 h (top) and at 6 h (bottom) following Bru labeling. RPKM reads/kilobase of transcript/million mapped reads. Representative examples of RNA transcripts destabilized (d) or stabilized (e) in sALS fibroblasts. Scatter plot (f) and gene ontology (g) for 333 transcripts showing a change in stability ≥1.5-fold in C9ALS fibroblasts, in comparison to control (Cntl) cells. Stability is calculated as a ratio of transcript abundance at 6 h vs. 0.5 h. Scatter plot (h) and gene ontology (i) for 324 transcripts showing a change in stability ≥1.5-fold in sALS fibroblasts, compared to control cells. FDR false discovery rate. Cell lines used for these experiments are listed in Supplementary Table 1