Fig. 2
From: A mosaic monoploid reference sequence for the highly complex genome of sugarcane

Sequencing strategy targeting the sugarcane monoploid genome based on the overall synteny and colinearity conservation within sugarcane hom(oe)ologs and with sorghum. a WGP sequence tags were produced from R570 sugarcane BACs. b WGP sequence tags were aligned onto the sorghum sequence, thus allowing the location of sugarcane BACs on sorghum. c A minimum tiling path of a BAC (MTP) corresponding to a monoploid sugarcane genome was defined and sequenced. d Overlapping BAC sequences were trimmed to construct the single tiling path (STP). e The STP sequence contains BAC contigs that belong to distinct hom(oe)ologous chromosomes. S. officinarum and S. spontaneum chromosome segments are represented in orange and red, respectively