Fig. 5 | Nature Communications

Fig. 5

From: Intermedin protects against sepsis by concurrently re-establishing the endothelial barrier and alleviating inflammatory responses

Fig. 5

IMD suppressed the recruitment of macrophages from bone marrow to the periphery via decreasing CCR2high cells. The bone marrow samples (a, d, g) and blood samples (j, m, p) from WT, IMD−/−, or IMD−/− mice rescued by IMD40 injection were analyzed using flow cytometry. Gating strategy: monocyte progenitors (MoP, identified as Ly6Chigh/CX3CR+); monocytes (Mo, identified as CD115+/CD11b+); macrophages (Mϕ, identified as F4-80+/CD11b+). Ratio of CCR2high (%) in BMMoP (b), BMMo (e), BMMϕ (h), blood MoP (k), blood Mo (n), and blood Mϕ (q) were quantified. The chemotaxis toward MCP-1 (CCL2) of the BMMoP (c), BMMo (f), BMMϕ (i), blood MoP (l), blood Mo (o), and blood Mϕ (r) were tested and quantified. Significance was assessed by Kruskal–Wallis test followed by non-parametric Dunn’s post-hoc analysis

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