Fig. 6
IMD alleviated the lung oedema and acute kidney injuries in sepsis. a Representative images show the HE-stained lungs. Lower panels: diagrams of the peri-vascular cuffs. The red arrows indicate the vessel area, and the yellow arrows indicate the cuff area. The ratio of peri-vascular cuff area/vessel area in a randomly chosen field was calculated using ImagePro Plus, and each data (the dot that was shown in the image) represents a mean value of five randomly chosen fields from one mouse, and the statistical data were calculated using six mice (n = 6). b The PHA-E-stained kidney samples. The red arrows point the brush borders, which indicate the thick microvilli-covered surface of proximal tubules. Lower panels: the measurement of the brush border thickness. c The ratio of lung cuffing area referred to the vessel area per lung (d) and the thickness of the brush border (pixels) per kidney were measured using Image-pro Plus and presented as plots ± SD. Each dot represents one mean level of five randomly chosen fields from one mouse, and the statistical data were calculated using six mice (n = 6). e–j After 9 h of CLP surgery, the blood concentration of BUN, CREA, ALT, AST, ALP, and TBIL was measured. Data were presented as scatter plots with mean ± SEM (n = 12). k The mRNA level of IMD in the liver relative to the control (Sham) were quantified. l–n The mRNA level of IMD in HUVEC (l), THP-1 (m), and peritoneal macrophage (n) were incubated in normoxia and hypoxia (1% O2). o The mRNA level of IMD in HUVECs treated with different doses of LPS. Significance was assessed by Kruskal–Wallis test followed by non-parametric Dunn’s post-hoc analysis (c–k, o) and t test (l–n). The control mice (WT or IMD−/−) was compared by Mann–Whitney test (c, d) or t test (e–j) particularly
