Fig. 2 | Nature Communications

Fig. 2

From: Designing a norepinephrine optical tracer for imaging individual noradrenergic synapses and their activity in vivo

Fig. 2The alternative text for this image may have been generated using AI.

FFN270 is a VMAT2 substrate. a VMAT2-dependent loading of FFN102 and FFN270 were compared using the normalized intensity of puncta (number of puncta multiplied by average fluorescence intensity ± SEM, n = 4 independent experiments) in VMAT2-HEK cells after a 2 h incubation (20 µM) under different conditions. FFN270 showed robust puncta intensity (581 ± 81), comparable to FFN102 (464 ± 49), that was significantly diminished in dTBZ (41 ± 4) and reserpine (44 ± 3) inhibited conditions (P < 0.0001, one-way ANOVA Dunnett test). b Representative schematic depicting the rationale for intracellular punctate fluorescence with active VMAT2, and general cytosolic labeling in null-transfected or inhibited conditions. The probes passively diffuse through the plasma membrane and are then actively concentrated in acidic compartments that express VMAT2. Representative images of FFN270 in c VMAT2-HEK cells, d dTBZ (2 µM) or e reserpine (2 µM) inhibited VMAT2-HEK cells, and f null-transfected HEK293 cells. Scale bar: 10 µm

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