Fig. 2 | Nature Communications

Fig. 2

From: p38-mediated phosphorylation at T367 induces EZH2 cytoplasmic localization to promote breast cancer metastasis

Fig. 2

p38 phosphorylates EZH2 in breast cancer. a Quantitative analysis of the direct interaction between recombinant EZH2 and p38α proteins using BioLayer Interferometry. EZH2 was immobilized on a sensor chip and a concentration series of p38α protein was added. Sensorgrams and corresponding fitting curves for kinetics constants and affinity determination (left) and corresponding plot of steady state response against concentration (right) for determination of binding affinity. b Western blot of MDA-MB-231 cells treated with 20 μM SB202190 for 48 hr (left panel) or with p38 shRNA (right panel) to pharmacologically and genetically inhibit p38 activity, respectively. c Western blot of MDA-MB-231 cells transduced with dox-inducible MKK6EE to activate p38α. d, e Immunohistochemical staining of 118 samples of human invasive breast carcinomas using anti-pEZH2(T367) and anti-H3K27me3 antibodies demonstrating a significant inverse association between these protein modifications (Chi-square, p < 0.00001) (×400 magnification)

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