Fig. 4
From: Single olfactory receptors set odor detection thresholds
Identification of TAARs that respond to phenylethylamine using DREAM. a Diagram of experimental workflow including odor exposure, isolation of olfactory mucosa, generation of cDNA, and measurement of receptor expression via qPCR. b, c Plots show relative levels of TAAR gene expression in the olfactory mucosa from odorized (green) and non-odorized (control; black) mice as determined by qPCR (normalized to the average non-odorized value). Mice were exposed to phenylethylamine at two concentrations for 24 h. Concentrations given are calculated values at the olfactometer outlet at the start of the experiment. Responsive TAARs are indicated by decreases in expression. Black lines displaying mean +/− SD are plotted over individual data points. Ventrally expressed TAAR genes (correspond to glomeruli outside the imaged area of the dorsal bulb) are shaded in pink. Asterisk denotes p < 0.01 General Linear Mixed Model
