Fig. 4

KIF20A works in coordination with the Ephrin-B–RGS complex in NPCs. a DNA plasmids were electroporated into the cortex at E13.5 and brains were analyzed at E15.5, E16.5, and E17.5. Distributions of transfected cells in the non-CP regions (IZ, SVZ, and VZ) were scored (5–8 electroporated brains for each plasmid or combination of plasmids were used for quantification). **P < 0.01 comparing rescue by shKif20a to controls. Error bars represent SD. Scale bar represents 100 μm. b Dissociated cells from the E13.5 cortices were co-infected with lentiviruses for expression of mCherry-RGS3/scrambled shRNA or mCherry-RGS3/shKif20a. After culturing for 2 days post infection, cells were fixed and stained with anti-KIF23/MKLP1 antibody. Percentage of telophase NPCs (with noticeable midbody localization of MKLP1, indicated by white arrows) showing midbody accumulation of mCherry-RGS3 was scored. **P < 0.01 (Student's t-test)