Fig. 5

Germline knockout of Kif20a causes embryonic lethality and loss of cortical NPCs. a Low recovery rate of homozygous Kif20a knockout first pups or embryos showed embryonic lethality due to loss of function of KIF20A. b At the peak of cortical neurogenesis (E15.5), brains from the surviving Kif20a homozygous mutant embryos were smaller than their wild-type littermates. c Nissl staining of brain sections revealed thinner cortices of the Kif20a homozygous mutant brains at E15.5. **P < 0.01. d Kif20a homozygous mutant brains had fewer βIII-tubulin⁺ neurons at E15.5. **P < 0.01. Scale bar represents 100 μm. e Kif20a homozygous mutant brains had fewer Pax6⁺ radial glial cells and fewer Tbr2⁺ intermediate progenitor or basal progenitor cells at E15.5. **P < 0.01. Scale bar represents 100 μm