Fig. 1
From: Programmed loading and rapid purification of engineered bacterial microcompartment shells
Comparison of different shell preparation methods. a SDS-PAGE analysis of HO shell preparations. Lane 1: Classic, Lane 2: in vivo capped, Lane 3: ex vivo capped. Shell protein identities indicated by arrows; loading normalized to A280 readings. b–d Negative stain TEM micrographs of three different shell preparations and corresponding structural models. Scale bar = 100 nm. e SDS-PAGE analysis of eluates from pentamer titration experiment. Lane 1: Ex vivo capped shells from Fig. 1a, Lanes 2–6: Titration of pentamers with equivalent volumes of each culture used in mixing experiments expressed in milliliters. f SDS-PAGE analysis of Halo carboxysome shell preparation using affinity purification. g Negative stain TEM micrographs of Halo carboxysome shell preparation. Scale bar = 50 nm. Results presented are representative of two independent biological replicates for a–d, f, and g. Findings in figure (e) recapitulate pilot experiments in which a subset of specified lysate ratios were used
