Fig. 6
From: Programmed loading and rapid purification of engineered bacterial microcompartment shells
Probe properties and permeability assay of uncapped and capped shells. a Schematic of probe behavior in presence of FlAsH and TEV protease. b Fluorescence emission spectra (excitation: 405 nm) of unencapsulated probe. c Emission spectra (450–600 nm) of encapsulated probe in uncapped and capped shells, in the presence of FlAsH and TEV protease. Axis numbers omitted for clarity; tick marks correspond to numbers in b. Results are representative of two independent technical replicates. Bacterial microcompartments are protein-bound organelles encapsulating segments of metabolic pathways. Here the authors functionalize shell proteins to facilitate facile purification and enable cargo encapsulation via covalent linkage
