Fig. 4

TIRR regulates 53BP1-dependent DSB response. a The mutations of TIRR disrupt the TIRR–53BP1 complex in vivo. TIRR, TIRR mutants, and 53BP1 were expressed in 293T cells. Co-IP and western blot were examined with indicated antibodies. b The mutations of TIRR do not affect the IRIF of 53BP1. Wild-type TIRR or its mutants were expressed in U2OS cells. The cells were treated with 10 Gy of IR. The IRIF of endogenous 53BP1 was examined by immunofluorescence staining. Foci positive cells were examined from three independent experiments with 100 randomly selected cells in each experiment. Scale bar represents 5 µm. c The mutations of TIRR do not affect the IRIF of RIF1. Data are represented as mean ± s.d. as indicated from three independent experiments. N.S.: non significant; ***: statistically significant (p < 0.001). Scale bar represents 5 µm