Fig. 2
Regulation of Serpine1 and miR-30 family members during serum stimulation. a Alignment of Serpine1-MRE with miR-30 family members. The seed-match (identical for all miR-30 family members) is shown in green, and the bases of 3′ complementarity, which is extensive only for two miRNAs (miR-30b, 9 nt; and miR-30c, 11 nt), in red. b Expression of Serpine1 upon serum stimulation of quiescent 3T9 fibroblasts. Left panel, RPKM. Right panel, contribution of Serpine1 expression to the total target pool, calculated over different classes of 3C-score. c–e Expression of miR-30 family members upon serum stimulation of quiescent 3T9 fibroblasts. Shown for each miRNA are: c the expression level (counts per million, CPM), d tailing events (% of 3′-non-templated isomiRs, 3NT) and e trimming events (% of trimmed forms, TRIM) in two independent experiments. f Frequency of tailing (3′NT isomiRs) for miR-30c-5p (top panel) or miR-30b-5p (bottom panel) in asynchronously growing 3T9 cells. Percentages of total reads. g Synthesis rate of the primary transcripts of the miR-30 family upon serum stimulation, as measured by a short pulse of 4SU. Definition of genomic regions and calculation of synthesis rate as copies per cell per hour (CPC/h) have been described previously27
